This technology is a mouse model that enables a precise flippase recombination in dopamine transporter (DAT)-expressing neurons that can be used to specifically target and study dopaminergic neurons.
Engineered mouse models have transformed neuroscience research by enabling cell-type-specific manipulation of gene expression and activity. Cre and flippase recombinases are widely used tools for conditional gene expression and deletion. However, their expression does not always reflect endogenous gene expression and can exhibit transgenerational variability, potentially affecting experimental outcomes.
This technology enables the precise introduction of flippase recombinase in dopamine transporter (DAT)-expressing neurons using a CRISPR-Cas9 gene-editing approach, allowing for the selective targeting of dopaminergic neuronal populations. Histological analyses and whole-cell electrophysiological recordings confirmed the high specificity and validated the excitability profile of this neuronal subpopulation. This model facilitates the investigation of dopaminergic neurons under both physiological and pathological conditions. This technology has been validated in in vivo mouse models.
IR CU26033
Licensing Contact: Kristin Neuman