A2a-FlpO mouse model for neuroscience studies
This technology is a set of knock-in mouse lines targeting basal ganglia circuitry and dopaminergic neurons for research studies.
Unmet Need: Precise cell-type-specific manipulation of neurons
Traditional methods for engineering transgenic mice typically rely on Cre and Flp recombinases, as well as bacterial artificial chromosome (BAC) constructs, to manipulate gene expression and activity. However, expression and regulation do not always correlate with endogenous expression, creating discrepancies that can arise from small variations leading to significant functional differences.
The Technology: Knock-in A2a-FlpO mouse model for basal ganglia circuitry and dopaminergic studies
This technology uses a knock-in strategy to engineer a series of five mouse lines with Cre or FlpO-mediated recombination in D1R, A2aR, and DAT-expressing dopaminergic neurons. This enables both cell-type-specific and precise manipulation of gene expression in basal ganglia neurocircuitry.
These mouse lines have been validated in expression and electrophysiological studies.
Applications:
- Drug discovery and screening
- Neuron-specific pharmacodynamics
- CNS disease modeling
- Basal ganglia circuitry profiling
- Behavioral neuroscience
Advantages:
- Cell-type specificity
- Orthogonal system to Cre for combinatorial genetic studies
- Precise gene expression regulation with knock-in model
Lead Inventor:
Related Publications:
Tech Ventures Reference:
IR CU26036
Licensing Contact: Cynthia Lang