Biofluid preparation method for chemical analysis

This technology is a multi-step method for preparing biofluids for mass spectrometry analysis using common laboratory chromatography materials.

Unmet Need: Simple and reproducible method for salt and protein removal from biofluids

Salt and proteins are removed from biofluid samples prior to mass spectrometry analysis. Methods for removal often include solvent precipitation and/or solvent-phase extraction, but these procedures require extensive hands-on time and can be costly. To implement mass spectrometry analysis in routine clinical settings, a straightforward, efficient sample preparation method must be established.

The Technology: Three-component column for biofluid separation

This technology is a sample preparation method that rapidly isolates target compounds from proteins, phospholipids, and salts in biofluids. A column is loaded with three components required for isolation: an affinity matrix, a metal oxide matrix, and a sorbent, which are layered and integrated into an automated flow path. The method uses commercially available single-use chromatography materials, demonstrating its accessibility and simplicity in clinical and laboratory settings.

Applications:

• Separation of components in biofluids
• Mass spectrometry analysis
• Quantitative drug level monitoring
• Metabolite monitoring
• Confirmatory toxicology
• Clinical diagnosis and treatment
• Food safety monitoring
• Environmental testing

Advantages:

• Simple sample preparation
• Inexpensive
• Uses commercially available materials
• Potential for high throughput
• Compatibility with automated liquid-handling systems

Lead Inventor:

Mazdak Bradberry, M.D., Ph.D.

Tech Ventures Reference:

• IR CU26156

• Licensing Contact: Jerry Kokoshka