Columbia Technology Ventures

DNA sequencing by synthesis with nucleotide analogues and Raman detection

This technology is a DNA sequencing approach that utilizes nucleotide analogues and Raman detection, reducing costs and increasing efficiency.

Unmet Need: Cost-effective method of DNA sequencing by synthesis

Current methods of DNA sequencing involve the use of expensive fluorophores that require constant time-consuming breaks for reads. There are currently no DNA sequencing assays that are fluorophore independent for use in high throughput sequencing.

The Technology: A fluorophore independent approach to DNA sequencing by synthesis

This technology utilizes Raman detection instead of expensive fluorophores for DNA sequencing by synthesis. Raman scattering is a photo scattering method that can identify nucleotides. The use of Raman spectroscopic signals for DNA sequencing by synthesis is an alternative for high-cost fluorophores in sequencing by synthesis approaches and allows scalability to high throughput sequencing.

Applications:

  • Sequencing for life sciences research
  • Personalized medicine
  • Genotyping services
  • Forensics

Advantages:

  • Increases efficiency
  • Cost-effective
  • High-throughput platform

Lead Inventor:

Jingyue Ju, Ph.D.

Patent Information:

Patent Status

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