This technology is a heterochiral DNA logic circuit that converts D‑DNA/RNA inputs to stable L‑DNA outputs via strand displacement, enabling nuclease‑resistant, bio-orthogonal sensing of natural signals.
DNA strand displacement currently serves as a powerful chemical framework for implementing enzyme-free molecular circuits. Previous studies have demonstrated its potential for intracellular biomarker sensing and for enabling diagnostic or therapeutic decision-making in vivo. However, a major obstacle to the application of these systems in cellular environments is the susceptibility of circuit components to nuclease-mediated degradation. Accordingly, there is a need for molecular circuit designs that maintain stability and resist degradation within cells.
This technology uses DNA-style logic circuits that take an input strand of one chirality (e.g., D-DNA) and, via two translator complexes, generate an output strand of the opposite chirality (e.g., L-DNA) via strand-displacement reactions. This heterochiral design makes the circuits resistant to endogenous D-nucleases and largely invisible to native nucleic-acid processes, while still allowing them to respond to natural D-DNA or RNA inputs.
This technology has been validated in vitro using a multistep leakless translator architecture.
Patent Issued (US 12,529,103)
IR CU19156
Licensing Contact: Jerry Kokoshka
