High-throughput single-cell multi-omics platform for integrated RNA, DNA, and protein analysis

This technology is a high-throughput single-cell multi-omics platform that integrates RNA, DNA, and protein analysis to enable comprehensive cellular profiling.

Unmet Need: Comprehensive, scalable, multi-omics single-cell analysis

Current single-cell analysis methods often focus on isolated molecular types, such as RNA or DNA, limiting their ability to provide a holistic view of cellular processes. Existing approaches for multi-omics analysis are constrained by low throughput, restricted to extracellular proteins, or lack scalability for large-scale studies. These limitations hinder comprehensive profiling of cellular heterogeneity and the integration of genetic, transcriptional, and proteomic data critical for understanding complex biological systems. Addressing these shortcomings enables deeper insights into cellular function, disease mechanisms, and therapeutic targets.

The Technology: Integrated single-cell RNA, DNA, and protein profiling platform

This technology enables high-throughput analysis of RNA, DNA, and proteins from individual cells by employing microfluidic chambers to co-encapsulate cells with specialized barcoded beads. These beads independently capture nucleic acids and proteins, allowing the integration of transcriptomic, genomic, and proteomic data for comprehensive cellular profiling. The platform addresses throughput challenges by leveraging scalable barcoding techniques and avoids fixation, preserving intracellular protein data for deeper biological insights.

Applications:

• Cell-type-specific drug discovery
• Personalized medicine
• Multi-omics analysis of tumors and tissues
• Research tools for disease mechanism studies
• Study of developmental processes in complex tissues
• Unbiased biomarker discovery
• Agricultural biotechnology
• Biologics production

Advantages:

• High-throughput single-cell platform
• Integrated platform allowing for RNA, DNA, and protein analysis
• Capable of intracellular and extracellular protein profiling
• Cost efficient
• Scalable
• Cell fixation not required

Lead Inventor:

Peter A Sims, Ph.D.

Patent Information:

Patent Pending (US20220403465)

Related Publications:

• Olsen TR, Talla P, Furnari J, Bruce JN, Canoll P, Zha S, Sims PA. “Scalable co-sequencing of RNA and DNA from individual nuclei.” bioRxiv [Preprint]. 2023 Feb 10.

Tech Ventures Reference:

• IR CU20054, CU21072

• Licensing Contact: Cynthia Lang