This technology is a set of polyyne-based optical materials and techniques for super-multiplexed imaging and spectral barcoding for molecule detection.
Current optical multiplexing techniques for simultaneous labeling of targets are fluorescence-based. This method limits the number of resolvable features and channels for simultaneous imaging. Optical barcoding is also highly dependent on the resolution of the detection method, since it requires a large quantity of unique optical barcodes. There is a lack of techniques that overcome the overlapping of fluorescence spectra to enable a higher number of targets and optical IDs.
This technology describes a library of optical materials for super-multiplexed imaging and spectral barcoding. It provides a set of Raman frequencies which improves color optical detection when combined with fluorescence microscopy. Additionally, this method achieves a high number of unique barcodes for optical readout through combinatory encoding. This technology can also include a dye-based system and further enhance multiplexing by modulating dye properties.
This technology has been validated in vitro in viral diagnostics studies.
Patent Pending (US20200199657)
IR CU17371, CU18220, CU22302, CU23284
Licensing Contact: Dovina Qu
