This technology is an open-source multichannel LED controller that precisely regulates the intensity and timing of high-power light sources and synchronizes with microscope scanning to enable artifact-free visual simulation and optogenetic activation during imaging.
Separation of fluorescence signals from optical stimulation during two-photon imaging is critical for capturing high-fidelity, low-noise neural data. Current approaches for presenting visual stimulation rely on optical filters, manual screening, or both, and offer only partial solutions, with residual artifacts appearing in the recorded movies. These methods lack precise synchronization between light stimulation and imaging acquisition, leading to interference between excitation, illumination, and detection. Addressing this gap is essential for enabling artifact-free integration of stimulation and high-resolution imaging.
This technology is a multichannel LED controller that enables precise, synchronized control of high-power light sources for visual stimulation and optogenetic activation. This system can precisely control up to eight LED outputs at currents up to 1.8 A with milliamp-level precision and minimal power ripple across the full operating range. Using a microsecond-accurate timing scheme, the system enables precise modulation via external analog signals and coordination with imaging workflows. This enables reliable integration of optical stimulation with high-resolution imaging.
This technology has been validated in vivo for optical stimulation and optogenetic applications in Drosophila and mouse models.
Patent Pending (US 20240142757)
IR CU23091
Licensing Contact: Kristin Neuman
