This technology is a recombinant protein tag that enhances the yield, solubility, stability, purification, and downstream applications of recombinant proteins that are generally difficult to produce.
Expression of recombinant proteins in bacteria with subsequent purification is a common method to produce biomolecules for biomedical applications. Currently, expression and purification of some recombinant proteins is significantly hampered by the formation of inclusion bodies, which consist of insoluble protein aggregates that require complex and often ineffective downstream processing to recover activity. This processing is time consuming, drastically limits effective yield, and poses a significant barrier to large-scale production of some recombinant targets.
This technology consists of a recombinant protein tag that prevents aggregation of the recombinant target protein in inclusion bodies and enhances downstream processing. The cationic tag partitions its recombinant partner to a phase separated condensate that can be easily recovered through conventional purification techniques with preserved biological activity of the target molecule. This technology enables the high-yield production of conventionally difficult recombinant protein targets in prokaryotes with preservation of biological activity.
This technology has been validated with single tagged recombinant protein.
Patent Pending
IR CU21175
Licensing Contact: Dovina Qu