This technology is a universal yeast donor strain to be used for the transfer of genetic materials between strains at a high-throughput level with almost 100% efficiency.
The controlled ability to produce yeast has several applications in the medical industry and brewing industry, from treating fungal infections to crafting recreational beer products. To develop Candida drugs and refine allotetraploid yeast strains for brewing processes, chemists first need to be able to transfer genomic material between yeast strains in an efficient and high-throughput manner. Current methods are limited by labor and time-consuming transformation protocols which introduce a bottleneck, and new, more efficient methods of DNA transfer between yeast strains are needed.
This technology is a universal donor strain that allows for the transfer of additional DNA fragments into new strain background at a high-throughput level. This process uses genetic material by mating then recovering haploid recipient without an intervening meiosis to achieve more efficient transfer. In diverse yeast strains, this described discovery could be used in manipulation of karyotypes in significant human pathogens that cannot undergo meiosis, or to foster development of antifungals. Unlike previous technologies, this method allows for efficient transfer of genetic material, does not require exogenous DNA, enables higher density screening, and is less laborious and time-consuming. Ultimately, this cost effective and robust technology may enable scalable manipulation of genetic material to promote innovation in the discovery of yeast-related drugs and brewing processes.
Patent Pending
IR 2308, 2902, CU12267
Licensing Contact: Joan Martinez