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This technology is an RNA templated genome editing technique that can be utilized in any organism, without the typical requirement for homologous recombination. Unmet Need: Genome editing without homologous recombination. Current CRISPR-based methods to edit the genome are dependent on homologous recombination, which may result in non-target deletions and insertions, translocations, and rearrangements.
Because only unmethylated CpG sites are modified, the technology facilitates alignment to the genome. “Large-scale structure of genomic methylation patterns.” Genome Res. DNA methylation at CpG sites, where a cytosine base is adjacent to a guanine, is a critical mechanism for regulating gene expression, maintaining genome stability, and dictating cell type during development.
Less Difficulty in Altering a Mouse Genome
In biomedical research, a very common technique is to alter a mouse's genome to induce or repress expression of a gene of interest. These two enzymes are rare eight base pair cutters and result in sticky ends upon digestion and can be used to excise the entire construct, for insertion into the plasmid with the ROSA26 genomic arms.