This technology describes an antibody that can be used in immunoassays to detect cells undergoing cell death via ferroptosis.
Ferroptosis is a recently discovered form of regulated cell death that underlies various disease states, including cancer and neurodegeneration. Current research methods for detecting cells in ferroptosis rely on markers of lipid peroxidation. However, there remains a need for a highly-specific and sensitive method of detecting and visualizing ferroptosis within cell cultures and tissue samples.
This technology describes a method for using immunostaining and flow cytometry assays to detect cells undergoing ferroptosis. The assay uses a primary antibody, termed 3F3 anti-Ferroptotic Membrane Antibody (3F3-FMA), to selectively label the human transferrin receptor protein 1 (TfR1), which serves as cell marker for ferroptosis. As such, this technology can be used to sensitively and specifically identify ferroptotic cells in numerous cell culture and tissue-based experimental contexts.
This technology has been validated in two human cell line xenograft cancer models, effectively and reliably staining tumor cells undergoing ferroptosis.
IR CU20038
Licensing Contact: Beth Kauderer