This technology is an aptamer-based graphene nanosensor that enables real-time detection and characterization of biomolecular interactions.
Current methods for detecting and monitoring biomolecules in a bodily fluid, such as assessing drug-target interactions or glucose levels, use indirect methods to monitor binding events or require difficult labeling techniques and complex instrumentation. As such, there is a need for a technology that directly characterizes biomolecular interactions over a wide range of sample conditions without losing specificity and sensitivity.
This aptamer-based graphene nanosensor identifies biomolecular interactions using a microfluidic field-effect transistor (FET). The FET is functionalized with analyte-specific aptamers that operate under varying ionic strength and temperature. Upon selective analyte enrichment and aptamer binding, the conductance of the graphene nanosensor changes and delivers an accurate and real-time electrical readout of the analyte activity. This technology enables direct, label-free characterization of complex biomolecular interactions.
This technology demonstrated the ability to quantitatively monitor insulin levels using IGA3 aptamer and has been used to evaluate the effect of sodium ions, magnesium ions, and variable temperatures on the binding kinetics of immunoglobin E with aptamer D17.4.
IR CU15154, CU17296
Licensing Contact: Dovina Qu
