This technology is an in vitro platform for the detection of gene transfer and recording temporal biological signals into the genomes of engineered cells.
CRISPR/Cas systems have recently been used to edit genetic material in different contexts. One of these applications is the record of biological signals in the genome of cells. However, there is a need for robust recording of temporally varying biological signals in living cells. Another of these applications is the detection of gene transfer but this require bioinformatic analysis or the expression of phenotypic markers. An easy-to-use and fast technology able to detect DNA transfer between cells would have a positive impact in the screening of gene delivery.
This technology is a CRISPR-based tool for detecting exogenous DNA transfer and recording a temporal biological signal in an engineered cell. It enables recognition of exogenous DNA delivery, and it records the presence of invading DNA in a bacterial cell, identifying its delivery for screening DNA-based therapeutics. This technology also permits the recording of biological events with high temporal resolution.
Patent Pending (US20220403419)
IR CU18063, CU20102, CU20160,
Licensing Contact: Cynthia Lang