DNA sequencing by synthesis with nucleotide analogues and Raman detection

This technology is a DNA sequencing approach that utilizes nucleotide analogues and Raman detection, reducing costs and increasing efficiency.

Unmet Need: Cost-effective method of DNA sequencing by synthesis

Current methods of DNA sequencing involve the use of expensive fluorophores that require constant time-consuming breaks for reads. There are currently no DNA sequencing assays that are fluorophore independent for use in high throughput sequencing.

The Technology: A fluorophore independent approach to DNA sequencing by synthesis

This technology utilizes Raman detection instead of expensive fluorophores for DNA sequencing by synthesis. Raman scattering is a photo scattering method that can identify nucleotides. The use of Raman spectroscopic signals for DNA sequencing by synthesis is an alternative for high-cost fluorophores in sequencing by synthesis approaches and allows scalability to high throughput sequencing.

Applications:

• Sequencing for life sciences research
• Personalized medicine
• Genotyping services
• Forensics

Advantages:

• Increases efficiency
• Cost-effective
• High-throughput platform

Lead Inventor:

Jingyue Ju, Ph.D.

Patent Information:

Patent Status

Related Publications:

• Fuller CW, Kumar S, Porel M, Chien M, Bibillo A, Stranges PB, Dorwart M, Tao C, Li Z, Guo W, Shi S, Korenblum D, Trans A, Aguirre A, Liu E, Harada E, Pollard J, Bhat A, Cech C, Yang A, Arnold C, Palla M, Hovis J, Chen R, Morozova I, Kalachikov S, Russo JJ, Kasianowicz JJ, Davis R, Roever S, Church GM, Ju J. “Real-time single-molecule electronic DNA sequencing by synthesis using polymer-tagger nucleotides on a nanopore array” Proc Natl Sci USA, 2016 May 10; 113(19): 5233-8.

Tech Ventures Reference:

• IR CU16261

• Licensing Contact: Cynthia Lang