This technology is cell line for detecting and tracking transmembrane protein interactions, including GPCR dimerization, within the plasma membrane of living cells to study protein dynamics and receptor interactions.
Current methods for studying GPCR interactions in live cells, including ensemble fluorescence techniques and single-particle tracking (SPT), struggle with low resolution and indirect interaction detection, often producing ambiguous results. These approaches cannot effectively differentiate between physical interactions and random proximity due to technical limitations in spatial and temporal resolution. Therefore, it is crucial to develop tools to regulate protein expression in a model amendable to single cell imaging to characterize GPCR dynamics.
This technology is a Chinese hamster ovary (CHO) cell line to precisely monitor and track single molecule interactions using single-molecule FRET (smFRET) imaging. This cell line uses inducible expression of self-labeling tags for site-specific covalent labeling. By combing bright, organic fluorophores this technology achieves high-resolution and real-time tracking without the need for potentially toxic photostabilizers. The platform addresses the limitations of conventional methods by providing direct measurements of receptor dynamics and interactions at a molecular scale, essential for advancing our understanding of GPCR functions. Validation testing has demonstrated its effectiveness in accurately detecting and analyzing receptor dimerization and conformational changes.
The technology has been validated using Chinese Hamster Ovary (CHO) cell lines.
IR CU24352
Licensing Contact: Jerry Kokoshka