High Resolution 3D Imaging of Purkinje Neurons
This technology is a protocol for generating 3D images of Purkinje Neurons across different layers of the cerebellum, at single cell resolution.
Unmet Need: Technique for selectively imaging single Purkinje neuron across the cerebellum
Purkinje cells are large neurons found in the brain, which are critical in controlling coordinated movement. Disruption of their function is therefore associated with a variety of neurodegenerative and neurodevelopmental diseases. Current methods of imaging these cells use immunofluorescence staining of a calcium binding protein. However, due to the high expression level of this protein in these cells, and their tightly layered distribution in the cerebellum, this method is not sufficient for resolving individual cells or 3D reconstruction.
The Technology: Protocol for high resolution 3D reconstruction of full Purkinje neurons
This protocol uses immunofluorescence co-staining to collect high resolution images of a Purkinje neuron across multiple cerebellum layers. One stain highlights the cell bodies, while the other improves resolution of the dendrites. These images can then be stitched together to form 3D reconstructions of the full, individual cells. These images can reveal subtle cell defects, or loss of cells, as is seen in diseased brain tissue.
This technology has been validated in a murine model.
Applications:
- Research tool for studying Purkinje neurons
- Research tool for preclinical neuro disease models
- Detection of subtle cell defects
- Imaging of other neuron types
Advantages:
- 3D reconstruction of cells
- Visualization of individual cells across cerebellum layers
- High resolution for resolving cell bodies and their dendrites
- Potential to be applied to a variety of cells
Lead Inventor:
Related Publications:
Tech Ventures Reference:
IR CU22200
Licensing Contact: Jerry Kokoshka