High-throughput metabolic profiling at single-cell resolution
This technology is a high-throughput and scalable platform for measuring the metabolic activity of single or bulk cells in drug screening and research applications.
Unmet Need: Scalable method for measuring the metabolic activity of individual cells
Current methods to measure metabolic activity require extensive sample preparation or extensive protocols. This limits the capacity for high-throughput metabolic profiling of large samples of individual cells. There remains a need for a cost-effective and scalable platform that is capable of evaluating metabolism at single-cell resolution, which would be highly useful for drug screening.
The Technology: Multiplexed vibrational probes for evaluation of single-cell metabolism
This platform combines spontaneous Raman spectroscopy with multiplexed vibrational probes to measure the metabolic activity of an individual cell or bulk of cells. Using an in-house micro-spectrometer and software package, this technology demonstrates the ability to distinguish among different cell lines at the single-cell level with high robustness and accuracy. Additionally, this technology can screen large libraries of compounds or genes within a few hours. This platform improves the ease and cost of sample preparation, thereby enabling high-throughput and large-scale metabolic profiling in drug screening applications.
Applications:
- Drug screening
- Platform for screening genes and other effectors of cell metabolism
- Single-cell metabolic profiling
Advantages:
- High-throughput
- Ease and low cost of sample preparation
- Measures metabolic activity at the single-cell level
- Rapid
- Scalable
Lead Inventor:
Patent Information:
Related Publications:
Tech Ventures Reference:
IR CU19155
Licensing Contact: Beth Kauderer