This technology is a miniaturized swept confocally-aligned planar excitation (SCAPE) microscopy platform called MediSCAPE that can be used for in-situ, label-free, real-time 3D imaging of living tissues without the need for excision of the target of interest.
Surgical procedures commonly require assessment of tissues or tumors during operation, yet these evaluations can take minutes to days due to the complex steps involved, such as excision, processing, slicing, staining, and imaging. This lag time increases costs and can be detrimental to the success of an operation. Although real-time imaging systems have been developed, many are large and cumbersome, increasing production costs and limiting their utility.
This platform is a miniaturized form-factor swept confocally-aligned planar excitation (SCAPE) microscopy platform, modified to improve utility and practicality. The system allows for in-situ, label-free, real-time 3D imaging of living tissues, without any excision of the tissues, using oblique light-sheet illumination. Cellular structures can be visualized in 3D with high sensitivity, and the system also tolerates in vivo movement.
This technology has been validated in vivo, with freshly excised mouse tissues and human kidney biopsies.
IR CU21147
Licensing Contact: Kristin Neuman
