This technology is a tissue culture method with controllable mimicry and patterning of the four phases of human skin wound healing (pre-injury skin, injured skin with stress-response, healing skin, and healed skin) using neonatal keratinocytes and fibroblast cells.
Wound healing is a complex biological process that can be broken down into four major stages; pre-injury, injury with stress response, healing, and healed. The most common method to model wound healing in-situ is the “scratch assay”, in which a cell monolayer is divided with a scratched line and allowed to migrate and fill the space. While current methods offer high throughput testing, these assays do not accurately capture all four stages of wound healing; therefore, more comprehensive high throughput models are needed to study wound healing and evaluate potential therapies to improve healing response.
This technology describes a cell culture protocol that mimics the four stages of human skin wound healing using a mixture of human neonatal keratinocytes and fibroblasts, termed skinoids. Importantly, the cells are transferred between 2D and 3D cultures to promote accurate modelling of the cellular environment at each stage. This model can be used as a research tool to study wound healing and the effects of therapeutics, cosmetics, and genetic defects on skin healing response.
IR CU24060
Licensing Contact: Sara Gusik