IncStim: A compact microscope for simultaneous neuronal imaging and optogenetic stimulation
This technology is a compact optogenetic imaging platform for real-time imaging, mapping, and stimulation of neuronal activity in 3D brain cell cultures and organoid models.
Unmet Need: Portable and affordable platforms for long-term neuronal imaging and stimulation
Current methods for monitoring and manipulating neuronal activity often rely on large, expensive, and technically complex imaging systems that are difficult to integrate into cell culture experiments. Existing platforms lack the ability to simultaneously image and precisely stimulate neurons in real time, especially in complex 3D neuronal cultures and organoid models. These limitations reduce accessibility, scalability and experimental flexibility for studying neurological diseases. There is a need for compact, cost effective, and integrated tools that enable long-term analysis and control of neuronal activity in vitro.
The Technology: Portable, integrated platform for neuronal imaging and stimulation
This technology is a compact microscope that enables simultaneous imaging and stimulation of neuronal activity within complex 3D neuronal cultures and organoid models. The platform combines a wide-field detection module with patterned light stimulation to allow real-time mapping and manipulation of neuronal networks. Its compact mechanical, optical, and electronic systems enable operation directly inside standard incubators, supporting long-term monitoring of brain cell behavior within cell cultures. The system also supports projector-based stimulation and integrated software for closed-loop control of neuronal activity with high spatial and temporal precision.
This technology has been demonstrated as a prototype platform for long-term neuronal imaging and stimulation experiments in vitro.
Applications:
- Neuronal activity mapping in 3D cell cultures and brain organoids
- Optogenetic stimulation and control of neuronal networks
- Disease modeling for neurological disorders
- Organ-on-chip monitoring and manipulation
- Long-term imaging of neuronal cultures in incubators
- Research tool for Neuroscience and machine learning research
- Closed-loop analysis of neuronal activity
Advantages:
- Enables simultaneous neuronal imaging and stimulation
- Supports real-time closed-loop monitoring and control of neuronal activity
- Incubator-compatible platform for experimentation
- Integrates imaging, stimulation, optics, and software into one system
- Portable and simplified mechanical and electronic design
- Compatible with 3D neuronal cultures and brain organoid models
- Enables continuous monitoring over long periods of time
- Potential to be applied to different disease models, improving overall accessibility for research
- Enables in vitro experimentation without direct human or animal testing, improving safety and reducing biological variability
Lead Inventor:
Patent Information:
Patent Pending(WO/2026/085204)
Tech Ventures Reference:
IR CU24391
Licensing Contact: Kristin Neuman