This technology describes a cell line that overexpresses glutathione peroxidase 4, making it resistant to lipid peroxidation and consequently ferroptosis.
Ferroptosis is a unique form of programmed cellular death which is dependent on cellular iron and the accumulation of lipid-based reactive oxygen species, particularly lipid hydroperoxides, making the process peroxidation-dependent. This process has been implicated in a diverse array of cancers and degenerative diseases, emphasizing its utility as a potential drug target for treatment of various diseases. However, the mechanism behind tumor sensitivity to ferroptosis is not well understood, and there is currently no high-throughput, robust method for screening potential therapeutics targeting this pathway.
This technology is an engineered cell line which is resistant to lipid peroxidation, and subsequently ferroptosis. Glutathione peroxidase 4 (GPX4) is a lipid repair enzyme that prevents ferroptosis by preventing lipid peroxidation. Therefore, overexpression of this protein results in cells that are resistant to lipid peroxidation, which consequently are resistant to ferroptosis. As such, this cell line can be used for studying the effects of compounds and factors that drive ferroptosis. By pairing this cell line with its parental version, it is possible to determine which compounds, genes and other factors act in a lipid-peroxidation-dependent manner.
IR CU20193
Licensing Contact: Joan Martinez
