Modular yeast biosensor for rapid pathogen detection

This technology is a visual biomarker to detect human, animal, and agricultural pathogens using bioengineered yeast.

Unmet Need: Rapid, effective diagnostic test for bacteria and other pathogens

There is a pressing need for sensitive and accurate diagnostic tests for bacteria and other pathogens. The majority of current diagnostic methods require specialized equipment and highly trained technical staff, which creates a prohibitive challenge to routine diagnostic testing in remote areas and in developing countries. As such, there is a need for a modular, low-tech diagnostic platform that may be easily deployed in resource-poor areas.

The Technology: Engineered baker’s yeast as biosensor for rapid pathogen detection

This technology is a method for rapid detection of human, animal, and agricultural pathogens. Bioengineered baker’s yeast express G protein-coupled receptors (GPCRs) that specifically detect peptide biomarkers produced by various pathogens with high sensitivity. When target peptides are present and bound, the yeast produce a red pigmentation visible to the naked eye. This provides a simple, reliable diagnostic test that does not require specialized equipment or trained staff. Additionally, the yeast biosensor may be easily dried for transportation and long-term storage. This technology provides a modular, low-cost diagnostic platform for pathogen detection that is ideal for resource-poor settings or resources that require routine monitoring for pathogens, such as water and wastewater treatment facilities.

This technology has been employed to successfully engineer yeast strains that produce lycopene in response to fungal pathogens in blood, urine, and soil samples.

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Applications:

• Rapid detection of bacterial and fungal pathogens
• Detection of agricultural pathogens
• Detection of biological warfare agents
• Routine monitoring of water and wastewater treatment facilities for pathogens
• Pathogen detection in resource-poor settings

Advantages:

• No training required to operate
• No need for laboratory or specialized equipment
• Can be use in remote or resource-poor settings
• Rapid results
• Easily detectable and reliable readout
• Yeast can be dried for transportation and long-term storage

Lead Inventor:

Virginia Cornish, Ph.D.

Patent Information:

Patent Status

Related Publications:

• Ostrov N, Jimenez M, Billerbeck S, Brisbois J, Matragrano J, Ager A, Cornish VW. “A modular yeast biosensor for low-cost point-of-care pathogen detection” Sci Adv. 2017 Jun 28;3(6):e1603221.

Tech Ventures Reference:

• IR CU1503

• Licensing Contact: Joan Martinez