Lead Inventor:
Frank Costantini, Ph.D.
Cell lineage tracing using living tissue of mice for study of genetic and developmental diseases:
Understanding development and cell lineage are key to combating genetic and developmental diseases. Thus there is a need for new model systems that allow the study of cell lineage. This technology provides a mouse strain that is useful for cell lineage tracing experiments. These mice will also be very useful for monitoring the expression of Cre in living tissues. Furthermore, by using modified forms of Cre whose recombinase activity is inducible, one can use these reporter mice to perform detailed analysis on the lineage of cells at different time points during development.
Cell lineage tracing in living tissue using transgenic mouse mouse strain:
This mouse strain expresses YFP from the ubiquitous ROSA26 locus promoter, after a transcriptional termination sequence, flanked by loxP sites, has been removed by Cre recombinase activity.
Applications:
• The study of cell lineage throughout development
• The study of Cre expression
Advantages:
• Easily visualized without recourse to the vital substrates required to visualize beta-gal in living tissue
Patent Status: Copyright
Licensing Status: Copyright / Material available for Express Licensing
YFP-Rosa26 Mouse Strain
Publications:
Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. Srinivas, S et al. (2001) BMC Dev Biol. 1:4.