This technology is a multiplex immunofluorescence method using beam-splitting imaging and non-specific antibodies to create a high-throughput, efficient approach for cell type identification.
Conventional immunohistochemistry (IHC) involves labeling a specific cell type with a corresponding fluorophore marker, typically used to image pathological tissue sections for diagnostic testing. There is a significant limitation in the number of cell types recognizable by IHC via chromogenic or fluorescent staining methods, as using too many fluorophores results in spectral overlap. Recent multiplex immunofluorescence (mIF) methods have enabled the simultaneous detection of multiple cells but remain limited by cross-interferences of intermediate immunostaining antibodies, labor-intensive processes, and prolonged imaging time.
This multiplex immunochemistry/immunofluorescence method, utilizing a beam-splitting imaging approach and non-specific antibodies, exponentially increases the number of identifiable cell types. By utilizing multiple primary antibodies for a specific fluorophore-binding secondary antibody, the potential number of identifiable cell types increases exponentially through this “combinatorial coding” approach. The 4-way spectral image splitter further enables simultaneous imaging of 4 colors with a reduction in aberrations and an increase in throughput and cell characterization.
Elizabeth M. C. Hillman, Ph.D.
Patent Pending(WO/2024/118888)
IR CU23161
Licensing Contact: Kristin Neuman