This technology is a high-speed 3D imaging platform that detects multiple fluorophores in biological specimens using improved swept confocally-aligned planar excitation (SCAPE) microscopy.
Confocal and two-photon microscopy are the leading approaches for imaging specimens for biomedical research. However, these technologies are costly, complex, and limited in their ability to image three-dimensional specimens at high speeds. These methods also have a low threshold of fluorophores they can detect in a given sample.
This technology utilizes swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed, multispectral imaging of 3D biological specimens at a high resolution. This technology can image intact and freely moving living samples at a much higher resolution than prior SCAPE systems while maintaining a large field of view. Moreover, this technology can perform high-speed spectrally multiplexed imaging, allowing one to detect more targets in each sample. This allows for improvements to resolution, throughput, field of view, penetration depth, and overall utility of the SCAPE system.
This technology has been validated in C. elegans worms.
Patent Pending (WO/2023/183516)
CU21252, CU21253, CU21254
Licensing Contact: Kristin Neuman
