Technologies
This technology is a trimethylamine oxide-supplemented medium to optimize the development of engineered cartilage in cell culture.
Though tissue engineering holds great promise for patients suffering from osteoarthritis, engineered cartilage typically lacks sufficient mechanical properties due to sub-native collagen levels. The media often used to grow cartilaginous tissue fails to promote collagen growth and maintenance.
This technology encompasses the use of trimethylamine N-oxide (TMAO) to enhance the properties of engineered cartilage tissue. TMAO is a natural osmolyte commonly found in various saltwater creatures, and has been shown to induce protein folding, helping to increase and preserve optimal collagen levels in tissues. Supplementation of tissue culture medium with TMAO is a simple method to enhance collagen content and mechanical properties of engineered cartilage in vitro.
The addition of TMAO to culture media has been shown to augment other collagen-enhancing approaches resulting in more functionally competent engineered cartilage grafts, and a dose-dependent increase in collagen content and mechanical strength were observed in cartilage grafts cultured in TMAO-supplemented media
IR 2823
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