This technology is a fully integrated holographic microscope system capable of simultaneous, high-resolution imaging and precise stimulation of 3D neuronal circuits using optogenetics.
Current neuro-imaging techniques often lack the capability to simultaneously image and stimulate neuronal circuits with high precision and resolution, especially in three dimensions, in vivo. Many systems face limitations in speed, spatial resolution, and flexibility to simultaneously stimulate and record from select groups of neurons. This technological gap hinders the detailed study and manipulation of complex neuronal networks in vivo, which remains crucial for understanding brain function and disorders. The proposed technology addresses these shortcomings by offering a fast, high-resolution, and flexible platform for both imaging and stimulation of 3D neuronal circuits.
This holographic module for microscopy integrates advanced holography for simultaneous stimulation and imaging of neuronal cells, in vivo. It features software-controlled remote focusing, point spread function sculpting, hologram power optimization, and adaptive optics. These capabilities allow for precise manipulation and observation of neuronal circuits in three dimensions with single-cell accuracy. The technology merges recent developments in holography, providing a unified solution for detailed and dynamic studies of neural circuits.
Patent Pending
IR CU23132
Licensing Contact: Kristin Neuman