This technology is a direct-tag method for rapid nanopore sequencing that enables rapid DNA extraction and high-yield sequencing of DNA fragments within a fraction of the time of current sequencing technologies.
DNA sequencing plays a vital role in prenatal and neonatal health screening, cancer diagnostics, as well as the detection of genetic diseases. However, current methods for DNA sequencing require large amounts of high-quality DNA, often require days to weeks to deliver results, and are very costly. These limitations delay critical genetic information to physicians, hindering important decisions for patient care. This underscores the urgent need for faster and more affordable DNA sequencing technologies.
This technology is a ligation-based sequencing barcoding system to provide reliable and high-throughput sequencing of DNA from a range of biological samples, such as tissue and amniotic fluid, without requiring extensive purification, quantification, and normalization steps. This technology optimizes DNA extraction from small amounts of DNA extracted from tissue using the Rapid Bead-beating-based (BBB) gDNA extraction which reduces the time for DNA extraction from overnight to 15min. This technology offers orders of magnitude improvement in processing speed and cost compared to current paradigms. As a result, this technology allows clinicians to make timely decisions for the course of treatment of patients and enables in-house sequencing to streamline basic research.
Patent Pending (EP3935164)
IR CU19163, CU19244
Licensing Contact: Cynthia Lang