Bacterial genetic engineering system to treat infections

The Technology: Modified BL21(DE3) E. coli strain with improved protein yield.

Development of new bacterial strains.

This technology is a modified bacterial model for improved expression and production of proteins.

CRISPR/Cas systems have recently been used to edit genetic material in different contexts.

“Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform” Nat Commun.

This technology is an in vitro platform for the detection of gene transfer and recording temporal biological signals into the genomes of engineered cells.

This technology describes a generalizable platform for screening and selecting CRISPR-Cas transcriptional activators specific to bacteria.

An identified CRISPR activator demonstrates the capacity to concurrently mediate activation and repression of transcription in E. coli, with multiplexing capabilities and portability to other bacterial species of commercial relevance.

This technology is a platform for identifying programmable and portable CRISPR-Cas transcriptional activators that function in bacteria.