Fluorescent labeling of cell surface proteins in vivo with spatiotemporal control
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Biochemistry & Molecular Biophysics
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Covalent bonds are stronger than non-covalent protein-protein or protein-small molecule interactions. This technology is an in vivo system in which a covalent bond can be formed between any two proteins in a cell-type specific manner for the purpose of immunoprecipitation, visualization, or modifying the activity of a target protein. This technology is an in vivo system in which a covalent bond can be formed between any two proteins in a cell-type specific manner for the purpose of immunoprecipitation, visualization, or modifying the activity of a target protein.
Most research in the life sciences requires protein based assays, underscoring the importance of characterizing protein modifications and protein interactions. Using a high throughput approach, the proposed method would enable researchers to identify unknown proteins using fluorescent dyes. This technology uses enzymes to break down peptide bonds and fluorescent dyes to label specific amino acids that are subsequently detected by their fluorescent intensity.
New tools to generate maps of binding sites for DNA-binding protein that can be tagged with fluorescent labels
Offers platform for developing automated image analysis. New tools to facilitate massively parallel data collection for single molecule analysis of protein-nucleic acid interaction, protein folding and unfolding, DNA dynamics. A robust and powerful platform portending massively parallel data acquisition of individual protein-nucleic acid interactions in real time
Perfect alignment of DNA molecules within the curtains facilitates data evaluation.
- 3Molecule
- 2Antibody
- 2Protein
- 1Amino acid
- 1Antigen
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- 3Chemistry
- 3Biochemistry
- 2Molecular biology
- 2Chemical classification
- 2Biomolecules by chemical classification
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- 1Eric C. Greene
- 1Peter A. Sims
- 1Richard S. Mann
- 1Siqian Feng
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- 2Cynthia Lang
- 1Jerry Kokoshka
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- 5Mechanical Engineering
- 5Medicine
- 5Psychiatry
- 3Biochemistry & Molecular Biophysics
- 3Biomedical Engineering
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- 3Columbia University Medical Center (CUMC)
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