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Covalent bonds are stronger than non-covalent protein-protein or protein-small molecule interactions. This technology is an in vivo system in which a covalent bond can be formed between any two proteins in a cell-type specific manner for the purpose of immunoprecipitation, visualization, or modifying the activity of a target protein. This technology is an in vivo system in which a covalent bond can be formed between any two proteins in a cell-type specific manner for the purpose of immunoprecipitation, visualization, or modifying the activity of a target protein. This technology is an advanced in vitro assay leveraging false fluorescent neurotransmitters (FFNs) to detect and quantify neurotransmitter leakage from synaptic vesicles, offering a dynamic and high-throughput method with implications for understanding neurological diseases and therapeutic developments. The Technology: Real-time assay for neurotransmitter leakage using fluorescent analogues. The technology employs HEK293 cells stably expressing human vesicular monoamine transporter 2 (VMAT2) and human SV2C, combined with a fluorescent dopamine analogue, FFN206. This technology describes the synthesis and characterization of fluorescent compounds that are substrates for monoamine transporters and can be used for direct imaging of neurotransmission at the synapse level. The Technology: Fluorescent false neurotransmitters for imaging neurotransmission. This technology identifies fluorescent compounds that accumulate in synaptic vesicles, allowing for direct visualization of neurotransmitter transport in individual dopamine terminals. 
