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DNA sequencing through fluorophore-free Raman spectroscopy of nucleotides. Next generation DNA sequencing methods typically increase throughput and coverage by decreasing the amount of DNA template used per reaction. Current DNA sequencing methods involve the attachment of fluorophores to each nucleotide and recording of the fluorescence signal that is produced upon excitation. Unmet Need: Cost-effective method of DNA sequencing by synthesis. There are currently no DNA sequencing assays that are fluorophore independent for use in high throughput sequencing. The use of Raman spectroscopic signals for DNA sequencing by synthesis is an alternative for high-cost fluorophores in sequencing by synthesis approaches and allows scalability to high throughput sequencing. The Technology: Combination of ion sensing and NRTs enables robust, inexpensive DNA and RNA sequencing. As such, this technology provides an inexpensive, efficient method for DNA and RNA sequencing with reduced error rates. Unmet Need: Efficient, cost-effective genome sequencing. 
